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Literature summary extracted from

  • Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3 (2010), Org. Biomol. Chem., 8, 3500-3508.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.332 expression in Escherichia coli Aspergillus fumigatus
1.3.1.100 expressed in Escherichia coli XL1 Blue MRF’ cells Aspergillus fumigatus
1.5.1.44 expressed in Escherichia coli XL1 Blue MRF cells Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.332 EDTA 485 residual activity Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.332 0.27
-
 chanoclavine-I pH 7.5, 37°C Aspergillus fumigatus
1.1.1.332 1.1
-
 NAD+ pH 7.5, 37°C Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.332 Mn2+ addition to EDTA-treated enzyme restores activity to 73% of initial activity Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.332 30000
-
 4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein Aspergillus fumigatus
1.1.1.332 118000
-
 gel filtration Aspergillus fumigatus
1.3.1.100 45000
-
 1 * 45000, SDS-PAGE Aspergillus fumigatus
1.3.1.100 46000
-
 gel filtration Aspergillus fumigatus
1.5.1.44 30000
-
 1 * 30000, His6-tagged enzyme, SDS-PAGe Aspergillus fumigatus
1.5.1.44 33300
-
 1 * 33300, calculated from amino acid sequence Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.332 Aspergillus fumigatus D3J0Z1
-
-
1.3.1.100 Aspergillus fumigatus V5IVA1
-
-
1.3.1.100 Aspergillus fumigatus B5233 V5IVA1
-
-
1.5.1.44 Aspergillus fumigatus
-
-
-
1.5.1.44 Aspergillus fumigatus Af293
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.100 Ni-NTA agarose column chromatography Aspergillus fumigatus
1.5.1.44 Ni-NTA agarose column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.332 chanoclavine-I + NAD+ incubation of enzyme with chanoclavine-I aldehyde in the presence of NADH does not result in detectable chanoclavine-I, indicating a much lower reaction velocity of the reverse reaction Aspergillus fumigatus chanoclavine-I aldehyde + NADH + H+
-
 ?
1.3.1.100 chanoclavine-I aldehyde + NADH + H+ in the presence of FgaFS only Aspergillus fumigatus dihydrochanoclavine-I aldehyde + NAD+
-
 ?
1.3.1.100 chanoclavine-I aldehyde + NADH + H+ in the presence of FgaFS only Aspergillus fumigatus B5233 dihydrochanoclavine-I aldehyde + NAD+
-
 ?
1.3.1.100 additional information the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH Aspergillus fumigatus ?
-
 ?
1.3.1.100 additional information the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH Aspergillus fumigatus B5233 ?
-
 ?
1.5.1.44 chanoclavine-I aldehyde + NADH + H+ the reaction occurs in the presence of the old yellow enzyme FgaOx3 Aspergillus fumigatus festuclavine + NAD+
-
 ?
1.5.1.44 chanoclavine-I aldehyde + NADH + H+ the reaction occurs in the presence of the old yellow enzyme FgaOx3 Aspergillus fumigatus Af293 festuclavine + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.332 tetramer 4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein Aspergillus fumigatus
1.3.1.100 monomer 1 * 45000, SDS-PAGE Aspergillus fumigatus
1.5.1.44 monomer 1 * 30000, His6-tagged enzyme, SDS-PAGe Aspergillus fumigatus
1.5.1.44 monomer 1 * 33300, calculated from amino acid sequence Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.332 fgaDH
-
 Aspergillus fumigatus
1.3.1.100 FgaOx3
-
 Aspergillus fumigatus
1.3.1.100 old yellow enzyme
-
 Aspergillus fumigatus
1.5.1.44 festuclavine synthase
-
 Aspergillus fumigatus
1.5.1.44 FgaFS
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.332 0.38
-
 chanoclavine-I pH 7.5, 37°C Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.332 additional information no cofactors: NADP+, FAD, FMN Aspergillus fumigatus
1.1.1.332 NAD+ specific for NAD+ Aspergillus fumigatus
1.3.1.100 FMN
-
 Aspergillus fumigatus
1.3.1.100 NADH
-
 Aspergillus fumigatus